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Updated: Sep 03, 2026
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ASCP ASCP-MLT Exam Overview:
| Certification Vendor: | ASCP Board of Certification (ASCP BOC) |
|---|---|
| Exam Name: | MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) |
| Exam Number: | ASCP-MLT |
| Available Languages: | English |
| Real Exam Qty: | 100 |
| Exam Format: | Computer Adaptive Testing (CAT), Multiple-choice questions |
| Related Certifications: | PBT(ASCP) MLT(ASCPi) MLS(ASCP) |
| Exam Duration: | 150 minutes |
| Exam Price: | $235 USD (standard); $215 USD (reduced for eligible regions) |
| Certificate Validity Period: | 3 years (renewable via CMP) |
| Passing Score: | 400 (scale 0-999) |
| Recommended Training: | ASCP Recommended Reading List ASCP BOC MLT Content Guideline |
| Exam Registration: | Pearson VUE Testing Center Scheduling ASCP BOC Official Application Portal |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Proctored computer-based testing at authorized Pearson VUE test centers |
| Pre Condition: | Associate degree or 60 semester hours from accredited institution + completion of NAACLS/ABHES-accredited MLT program OR approved education + clinical experience; multiple eligibility routes available |
| Official Syllabus URL: | https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT |
ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Transfusion reactions and adverse events - Antibody detection and identification - Blood group systems and antigens - Compatibility testing |
| Topic 2: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Electrolytes, acid-base balance, and blood gases - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests |
| Topic 3: Microbiology | 15-20% | - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility - Mycology, parasitology, and virology |
| Topic 4: Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes - Laboratory safety and regulatory compliance - Quality control and quality assurance |
| Topic 5: Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis - Cerebrospinal, serous, and synovial fluids - Urine formation and composition - Semen and amniotic fluid testing |
| Topic 6: Hematology | 20-25% | - Routine and automated hematology procedures - Red blood cell physiology and disorders - Hemostasis and coagulation testing - White blood cell morphology and diseases |
| Topic 7: Immunology and Serology | 5-10% | - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders - Immune system principles and components - Infectious disease serology |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
A quality control program is to be set up for the following tests:
Phenylalanine deaminase (PAD)
Indole production
Voges-Proskauer (V-P)
Which pair of stock culture organisms would be MOST suitable to verify the performance of these three tests?
A. vulgaris, Escherichia coli Klebsiella pneumonia, Proteus vulgaris
B. coli, Enterobacter aerogenes
C. aerogenes, K. pneumonia Bacillus anthracis, Yersinia pestis
Question 2
A monoclonal B-cell population (Kappa or Lambda predominant) with expression of CD19, CD20, CD23, and co-expression of CD5 is consistent with which of the following?
A. Mantle cell lymphoma
B. Chronic
C. A normal B cell
D. A mature T cell
Question 3
PCT usually rises within 3-6 hours of infection. CRP also increases rapidly following infection, but is not as specific for infection as PCT. A rise in CRP could also occur with SIRS. Lactic acid is usually used to detect and monitor impaired circulation and tissue oxygenation in critically ill patients.
Chemistry
Of the three laboratory tests that are listed, which has proven to be most effective for early differentiation of systemic inflammatory response syndrome (SIRS) from sepsis due to its increase following infection and higher specificity?
A. Lactic acid
B. C-reactive protein (CRP)
C. Procalcitonin (PCT)
Question 4
A regional laboratory that performs high volume, highly complex or infrequently ordered testing is a:
A. long-term care facility.
B. physician's office.
C. wellness clinic.
D. reference laboratory
Question 5
Creatinine clearance tests are utilized to estimate the glomerular function of the kidney. The creatitine clearance calculation is defined as the volume of plasma that is cleared of creatinine by the kidney per unit of time and uses the following formula:
creatinine clearance = Urine creatinine conc x Volume / Plamsa creatinine conc Creatinine clearance measurements have a recommended specimen requirement = 24-hour urine collection.
Which of the following tests would be useful in the assessment of glomerular filtration:
A. Creatinine clearance
B. 24 hour urine protein
C. PSP test
D. Urea clearance
Solutions:
| Question 1 Answer: A | Question 2 Answer: B | Question 3 Answer: C | Question 4 Answer: D | Question 5 Answer: C |
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